ISO 16649-1:2001

Resuscitation: inoculation of a specified quantity of the test sample or initial suspension on to cellulose acetate membranes overlaid on mineral-modified glutamate agar (MMBA), then incubation at 37°C (or 30°C) for 4h.

Inoculation on additional same agar with the same conditions of decimal dilutions from the test sample or the initial suspension.

Isolation: transfer of membranes from the resuscitation stage on the mineral-modified glutamate agar to tryptone-bile glucuronide agar (TBX). Incubation at 44°C for 18-24h.

Enumeration: calculation of the number of CFU of presumptive Eschericha coli per gram or per millilitre of sample from the number of typical blue CFU.

 

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