Nota Applicativa Microbiologia N.10 – Notizie utili di microbiologia anaerobica

Question 1

How is important the use of specialized anaerobic media?

Answer:
1. Media like Columbia Tryptic Soy, Wilkins Chalgren and Schaedler are unsuitable.
2. Two media are better that one because many anaerobic infections  contain more than one type of anaerobe.
3. Incubation should be for a minimum of 48 hours and continue until at least 5 days.
4. The common selective media:

Genus/Group	Media for use	Concentrations
Anaerobic cocci	Nalidixic acid Tween 80	10mg/L 0,1%
Anaerobic GNRs	Nalidixic acid Vancomycin	10mg/L 2,5mg/L
Clostridia	Neomycin	100mg/L
Actinomyces	Nalidixic acid metronidazole	30mg/L 10mg/L

 

Question 2

Which are the common reasons for the failure of anaerobiosis?

Answer:
– Anaerobe jar leakage
- Poor catalyst activity
- Failure to use biological and chemical indicators
- Wrong biological indicator
- Failure to include CO2 in the anaerobic atmosphere
- The so-called “five o’clock build up”: waiting until the end of the day before incubating anaerobic plates.

  

Question 3

Which are the common reasons for the failure of anaerobiosis?

Answer:
- Anaerobe jar leakage
- Poor catalyst activity
- Failure to use biological and chemical indicators
- Wrong biological indicator
- Failure to include CO2 in the anaerobic atmosphere
- The so-called “five o’clock build up”: waiting until the end of the day before incubating anaerobic plates.